1Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
2Department of Life Sciences Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
چکیده
Cancer is one of the most common health and mortality problems in the modern world. Lung cancer is a disease characterized by the uncontrolled growth of cells in the lung tissue. Researchers are now looking for new methods and appropriate treatments for cancer. Nanobiotechnology is one of the tools that is considered an influential factor in promoting proper cancer treatment. Nanoscience can change the properties of biomaterials, including their stability, biocompatibility, and solubility. In the design of carriers of drug delivery systems, the goal is to achieve a system with proper drug loading and desired release properties with a high half-life and low toxicity. One of these carriers is a liposome. Liposomes are spherical, and liposomes are Composed of the lipid bilayer. Their most important feature is the accurate delivery of the drug to the target tissue, and they can also accommodate large amounts of the drug. Herbal medicines are used in the treatment of cancer due to their unique properties and antioxidant properties. Curcumin, an active ingredient of turmeric, has anti-cancerous and antioxidant properties, but it is unstable and has a slight solubility in water. Therefore, curcumin's stability and solubility can be enhanced by encapsulating it in drug carriers such as liposomes. In this study, nanoliposomes containing curcumin were designed using different formulations. Curcumin-containing nanoliposomes were fabricated using the thin layer hydration method. Then, the process of drug delivery, drug release, and its effect on lung cancer cells was evaluated. Curcumin-loaded liposome diameter decreased with increasing sonication amplitude and with filtration. The formulation of nano-liposomes sonicated at 60% amplitude showed a narrow size distribution with an average diameter (Number) of 97nm, and the encapsulation efficiency for curcumin was about 74.27%. Curcumin-containing nanoliposomes were affected on lung cancer cell line A549. It has also demonstrated that these Nano-preparations have no cytotoxicity effects on normal cells. This study's overall result indicates that the type and amount of phospholipids influenced the preparation of the optimal liposomal formulation containing curcumin.
